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miRNA Primer Synthesis

miRNA primer design is based on the mature miRNA sequence and utilizes a stem‑loop structure to enable specific reverse transcription and subsequent amplification. The stem‑loop RT primer is approximately 45 bp in length and is capable of forming a stable hairpin structure.

Bisulfite sequencing PCR (BSP) Primer Synthesis

Bisulfite sequencing PCR (BSP) primers are designed as a pair of 25–30 bp oligonucleotides that do not contain any CpG dinucleotides. After bisulfite treatment of genomic DNA, all unmethylated cytosines (C) are converted to uracils (U), while methylated cytosines remain unchanged. During PCR amplification, uracils introduced by bisulfite conversion are read as thymines (T), enabling direct detection of methylation status through sequencing.

Methylation‑specific PCR (MSP) Primer Synthesis

Methylation‑specific PCR (MSP) requires primers that can distinguish between methylated and unmethylated DNA after bisulfite conversion. An MSP primer pair typically includes 3–5 potential methylation sites within the amplified region.

ddUTP·Na3,100mM solution

ddTTP·Na3,100mM solution | 84445-38-5

ddGTP·Na3,100mM solution | 68726-28-3

ddCTP·Na3,100mM solution | 66004-77-1

ddATP·Na3,100mM solution | 132619-65-9

5-ap-ddUTP·Na3 | 114748-59-3

7-ap-7-Dazae-ddGTP·Na3 | 114748-61-7

5-ap-ddCTP·Na3 | 114748-56-0

7-ap-7-Dazae-ddATP·Na3(100mM solution) | 114748-69-5

7-ap-7-Dazae-ddATP·Na3 | 114748-69-5

7-ap-7-Deaza-2'-dGTP·Na3, 100mM solution | 587848-73-5

7-ap-7-Deaza-2'-dATP·Na3, 100mM solution | 587848-72-4

5-ap-2'-dUTP·Na3, 100mM solution | 179101-49-6

5-ap-2'-dCTP·Na3, 100mM solution | 115899-39-3

7-ap-7-Deaza-2'-dATP·Na3 | 587848-72-4

3'-O-azidomethyl-dTTP·Na3, 100mM solution

3'-azidomethyl-dTTP.4Li, 5mM solution